Human Mesenchymal Stem Cell (hMSC) cultures involve isolating multipotent cells from tissues like bone marrow, fat, or umbilical cords, expanding them in vitro, and directing their differentiation into bone (osteocytes), cartilage (chondrocytes), fat (adipocytes), and other cells, using specialized media and conditions, crucial for regenerative medicine, tissue engineering, and therapy due to their self-renewal and immunomodulatory properties.
Key Aspects of hMSC Cultures:
Sources:
Common sources include bone marrow (BM-MSCs), adipose tissue (AD-MSCs), and umbilical cord/Wharton’s Jelly (UC-MSCs), each with slightly different differentiation potentials.
Characteristics:
They are fibroblastic, adhere to plastic, and must meet International Society for Cellular Therapy (ISCT) criteria (positive for CD105, CD73, CD90; negative for lineage markers).
Culture Media:
Optimized media, often serum-free or xeno-free, support their proliferation and maintain their stemness, with specific differentiation media used for lineage commitment.
Differentiation:
Can be induced to form bone, cartilage, fat, muscle, neurons, and heart cells, depending on the specific medium and conditions.
Applications:
Used in regenerative medicine for treating injuries, autoimmune diseases, liver conditions, and in tissue engineering, requiring large-scale, consistent production.
For more details, contact our team at bd@cell-bio.life
